Part:BBa_J176131:Design

PLrigid

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 20
  Illegal PstI site found at 35
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 20
  Illegal PstI site found at 35
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 20
  Illegal PstI site found at 35
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 20
  Illegal PstI site found at 35
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

• The peptide sequence is repetitive [EAAAR][EAAAR][EAAAR][EAAAR] (Yan et al., 2007). In order to reduce repetition at the DNA sequence level, human-optimazed codons were distributed randomly along the sequence. This was done using a permutation tool I found online, along with some tedious manual text editing to create more variation where the sequence still looked too repetitive
• De novo DNA synthesis was provided by Genscript

Source

TBA

References

1. Yan W, Imanishi M, Futaki S, Sugiura Y. (2007) alpha-Helical Linker of an Artificial 6-Zinc Finger Peptide Contributes to Selective DNA Binding to a Discontinuous Recognition Sequence. Biochem. 46: 8517-8524